Differences Between Enzymatic and Diazo Methods for Measuring Direct Bilirubin
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چکیده
منابع مشابه
Ultramicro method for serum bilirubin by diazo blue reaction.
ROPER CLINICAL MANAGEMENT of infants with erythroblastosis fetalis necessitates the determination of total serum bilirubin. Of the many modifications of the original diazo reaction on blood serum (1) a micromethod using capillary blood is preferable. One tenth miffiliter of serum was used by Davidson et al. (2) in their modification of the Ernst and F#{246}rster (3) method and by With (4, 5) in...
متن کاملLight has a greater effect on direct bilirubin measured by the bilirubin oxidase method than by the diazo method.
We compared the effect of light on direct-reacting bilirubin (DBIL) measurement by the bilirubin oxidase (EC 1.3.3.5; BOX) method and by the Jendrassik-Gróf diazo method. DBIL concentrations determined by the BOX method in the sera of hyperbilirubinemic infants treated with phototherapy yielded falsely higher values than those by the direct diazo method. A similar tendency was noted when DBIL c...
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A new technic for the determination of total bilirubin is reported. The substitution of 2,4 dichloroaniline for sulfanilic acid results in a very stable diazotized reagent which reacts quickly with bilirubin in plasma or serum. Comparison with a sulfanilic acid procedure indicates that similar analytic values are obtained with this technic; short quality control studies indicate satisfactory pr...
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Bilirubin in Newborns: Comparison with HPLC and an Automated Diazo Method, Steven C. Kazmierczak, Alex F. Robertson, Paul G. Catrou, Kimberly P. Briley, Bill L. Kreamer, and Glenn R. Gourley ( Department of Pathology, Oregon Health and Sciences University, 3181 SW Sam Jackson Park Rd., Portland, OR 97201; Departments of 2 Pediatrics and 3 Pathology and Laboratory Medicine, Brody School of Medic...
متن کاملDirect bilirubin higher than total bilirubin?
Both total and direct bilirubin measure the formation of azobilirubin, which is detected at 570/660 nm. The total bilirubin reaction contains surfactant and caffeine, which solubilize “indirect” bilirubin and accelerate the reaction (1). Direct bilirubin is a component of total bilirubin and therefore should always be smaller in value. The presence of a monoclonal immunoglobulin (2220mg/dL) fal...
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ژورنال
عنوان ژورنال: Clinical Chemistry and Laboratory Medicine
سال: 1995
ISSN: 1434-6621,1437-4331
DOI: 10.1515/cclm.1995.33.8.513